SIMPLE SCREENING FOR POTENTIAL CHRYSENE DEGRADING FUNGI

Cultivation of Hydrocarbon-Degrading Fungi

Fungi form the biggest microbial biomass and are key drivers of hydrocarbon biodegradation. We here describe standard cultivation methods for fungi that use hydrocarbons either as sole carbon and energy source or co-metabolically degrade them. The methods include both liquid (Protocols 1 and 2) and solid-state cultivation approaches (Protocol 3) as well as a versatile protocol to monitor fungal...

Isolation and Screening of Cellulose Degrading Bacteria in Kitchen Waste and Detecting Their Degrading Potential

The present paper investigates cellulose degrading bacteria present in kitchen waste for highest cellulase activity and growth of bacteria at optimum temperature and pH. Sample was collected from domestic kitchen waste and bacterial strains were isolated using nutrient agar media. Enrichment technique was used for isolating CDB (cellulose degrading bacteria) strains. The objective of research w...

Endophytic Fungi, Characteristics and Their Potential for Genetic Manipulation

A Simple and Efficient Dye-based Technique for Rapid Screening of Fungi for L-asparaginase Production

Three hundred and sixty four (364) isolates of tropical soil fungi were screened for L-asparaginase production by rapid plate method using modified Czapek-Dox agar containing L-asparagine, and either bromo cresol purple or phenol red dye as an indicator. Results of the study revealed that total one hundred and thirty five (135) isolates showed positive reaction for L-asparaginase production as ...

Screening of Lovastatin Production by Filamentous Fungi

In the present study, 110 fungal strains of Persian Type Culture Collection (PTCC) including some selected strains isolated in various screening projects were tested for their potentiality to produce lovastatin, a competitive inhibitor of 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reduc-tase), the rate-limiting enzyme of cholesterol biosynthesis. The fungal strains were cultivated in a t...